Ampia gamma di anticorpi secondari ad alta AFFIDABILITA', RIPRODUCIBILITA' e STABILITA'.
Con oltre 7.000 anticorpi secondari Whole IgG, Fab e F(ab')2 nel nostro database troverai sempre la giusta combinazione di coniugati specifici per il tuo saggio immunologico. La selezione comprende anticorpi secondari coniugati con horseradish peroxidase o alkaline phosphatase per Western Blotting (WB), ELISA e Immunohistochemistry (IHC) nonché anticorpi secondari coniugati per metodi basati su fluorescenza come Immunofluorescent Staining e Flow Cytometry (FACS). Le coniugazioni fluorescenti includono una selezione di fluorofori Alexa ™, Cynanin ™ (Cy2, Cy3 e Cy5) e DyLight® 405.
Choosing the right affinity-purified secondary antibody for your application
Affinity-purified antibodies are isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. A proprietary elution process is used to dissociate antibodies from the antigen. Unconjugated affinity-purified antibodies are supplied sterile filtered in phosphate buffer without stabilizers or preservatives. Conjugated affinity-purified antibodies are freeze-dried in phosphate buffer with stabilizers and sodium azide, with the exception of horseradish peroxidase conjugates, which do not contain a preservative.
Affinity-purified secondary antibodies are offered in three different forms: whole IgG, F(ab') 2 fragments, and Fab fragments.
The antibodies are listed alphabetically according to the host species of the antigen (i.e. the primary antibody). For example, if the primary antibody is made in mouse, select "Anti-Mouse". This is the target species in the product filter.
Note: Both anti-Syrian and anti-Armenian hamster secondary antibodies are listed under "Anti-Hamster". It is important to know in which strain of hamster the primary antibody was produced in order to select the proper secondary antibody, since cross-reaction between the strains is not complete.
Selection of the host species for a secondary antibody involves many considerations, including but not limited to:
Note: Immunoglobulins from different species share similar structures, with similarities being related to closeness in phylogeny. Antibodies against immunoglobulins from one species may cross-react with a number of other species unless they have been specifically adsorbed against the cross-reacting species. Antibodies that have been adsorbed against other species will contain "(min X...Sr Prot)" in the antibody description.
Selection of fluorophores depends on:
Effective 4-color imaging with good color separation, good photostability, and high sensitivity is possible with combinations such as DyLight 405, Alexa Fluor® 488, Rhodamine Red-X, and Alexa Fluor® 647.