Test di laboratorio innovativi per valutare la qualità del seme umano e animale
SPQI sviluppa e produce con il marchio 4BioDx anticorpi monoclonali anti-AKAP4 e kit ELISA specifici per studiare la funzionalità degli spermatozoi umani e animali.
AKAP4 gioca un ruolo centrale nella struttura flagellare, nella chemiotassi, nella capacità e nella motilità degli spermatozoi (Luconi et al., 2011).
AKAP4 (chiamato anche AKAP82) è uno dei componenti principali (~ 50%) della guaina fibrosa del flagello spermatico. Il precursore AKAP4 definito pro-AKAP4 viene trasformato nella maturazione a AKAP4.
La perdita dell’espressione di AKAP4 porta a malformazioni di flagelli spermatici, quindi immotilità e sterilità (Miki et al., 2002).
La concentrazione di proAKAP4 è un buon indicatore della qualità dello sperma e di come possano gli spermatozoi mantenere la loro motilità alta ed efficiente fino alla fecondazione.
Il dosaggio del biomarcatore proAKAP4 fornisce:
- Informazioni obiettive sulla qualità dello sperma
- Parametro pertinente per migliorare la crioconservazione dello sperma
- Qualificazione di ciascun maschio riproduttore e del suo eiaculato
- Indicatore per selezionare il miglior maschio riproduttore
Pro-AKAP4 ELISA kits
Il kit pro-AKAP4 ELISA è un kit ELISA completo che include una micropiastra pre-coated e tutti i reagenti e i tamponi necessari per quantificare la proteina pro-AKAP4 nello sperma.
Principali vantaggi del kit ELISA proAKAP4:
- Funziona sia con eiaculato fresco sia con seme refrigerato o congelato
- Occorrono pochi microlitri: da 20/25 a 40/50 μl
- Immunoassay facile da usare
- Per procedure manuali e automatizzate
- E’ uno strumento obiettivo per classificare eiaculati animali
- Monitoraggio durante la fertilità (età, quarantena, etc)
- Indicativo della qualità del materiale refrigerato nel tempo
- Misura 40 duplicati di sperma in meno di 2 ore
4VDX-18K4 Bovine 4MID proAKAP4 ELISA Kit
4VDX-19K11 Camel 4MID proAKAP4 ELISA Kit
4VDX-19K10 Cat 4MID proAKAP4 ELISA Kit
4VDX-18K5 Dog 4MID proAKAP4 ELISA Kit
4VDX-18K9 Goat 4MID proAKAP4 ELISA Kit
4VDX-18K3 Horse 4MID proAKAP4 ELISA Kit
4VDX-18K8 Mouse 4MID proAKAP4 ELISA Kit
4VDX-18K2 Pig 4MID proAKAP4 ELISA Kit
4VDX-18K6 Rabbit 4MID proAKAP4 ELISA Kit
4VDX-18K7 Ram 4MID proAKAP4 ELISA Kit
Monoclonal Antibodies against AKAP4 biomarker
Gli anticorpi monoclonali contro AKAP4 sono adatti per le applicazioni di immunofluorescenza mediante microscopia classica e analisi CASA (Computer-Assisted Sperm Analysis) per valutare la morfologia dello sperma e la motilità degli spermatozoi.
L’anticorpo monoclonale anti-AKAP4 murino (Rif. 4BDX-1602) riconosce la regione carbossi-terminale della proteina di ancoraggio A-chinasi 4 (AKAP4).
L’anticorpo 4BioDX anti-pro AKAP4 (Rif. 4BDX-1701) riconosce specificamente il precursore della proteina di ancoraggio di A-chinasi 4 (pro-AKAP4).
4BDX-1602 Anti-AKAP4 C-terminus (clone 7E10)
4BDX-1701 Anti-pro AKAP4 Prodomain (clone 6F12)
4BDX-1901 Anti-proAKAP4 Prodomain (clone 5H2)
4BDX-1805 Anti-AKAP4 C-terminus (clone 10F8)
Main scientific references that are using the proAKAP4/AKAP4 antibodies and 4MID® Kits from SPQI S.A.S:
2023
Dordas-Perpinyà M, Sergeant N, Yanez-Ortiz Y, Catalan J, Delehedde M, Bruyas JF, Briand-Amirat L and Miró J (2023) Characterization of proAKAP4 expression, metabolism and localization in Equus asinus spermatozoa. Reproduction, Fertility and Development. Vol. 35(2):215-216. https://doi.org/10.1071/RDv35n2Ab176
Duracka M, Benko F and Tvrdá E (2023) Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability (review). Int. J. Mol. Sci. Vol. 24:3379. (review) https://doi.org/10.3390/ijms24043379
Gardela J, Ruiz-Conca M, Palomares A, Olvera-Maneu S, García-Calvo L, López-Béjar M, Martínez-Pastor F and Álvarez-Rodríguez M (2023) Effect of Honey, Coenzyme Q10, and-Carotene-Tocopherol as Novel Additives in Rabbit-Sperm Cryopreservation Extender. Animals Vol. 13(14):2392. https://doi.org/10.3390/ani13142392
Nixon B, Schjenken JE, Burke ND, Skerrett-Byrne DA, Hart HM, De Iuliis GN, Martin JH, Lord T and Bromfield EG (2023) New horizons in human sperm selection for assisted reproduction. Front. Endocrinol. 14:1145533. (review) https://doi.org/10.3389/fendo.2023.1145533
Oh EY, Chandanee MR, Dissanayake WMN, Lee SM and Yi YJ (2023) Comparison of fertility competence in toll-like receptor 4 (TLR4)-knock out male mice fed a high-fat diet. J Biomed Transl Res Vol. 24(2):41-52. https://doi.org/10.12729/jbtr.2023.24.2.41
2022
Boersma A, Primus J, Wagner B, Broukal V, Andersen L, Pachner B, Dahlhoff M, Rülicke T and Auer KE (2022) Influence of sperm cryopreservation on sperm motility and proAKAP4 concentration in mice. Reprod Med Biol. Vol. 21:e12480. https://doi.org/10.1002/rmb2.12480
Bustamante-Filho I, Pasini M and Moura AA (2022) Spermatozoa and seminal plasma proteomics: too many molecules, too few markers. The case of bovine and porcine semen. Animal Reproduction Science. Vol.247:107075. https://doi.org/10.1016/j.anireprosci.2022.107075
Carracedo S, Briand-Amirat L, Dordas-Perpinyà M, Ramos Escuredo Y, Delcombel R, Sergeant N and Delehedde M (2022) ProAKAP4 protein marker: towards a functional approach to male fertility. Animal Reproduction Science. Vol.247(107074):1-20.https://doi.org/10.1016/j.anireprosci.2022.107074
De Almeida MAB, Megumy Tsunokawa Hidalgo M, Zito de Moraes FL, Corsi Trautwein LG, Schnitzer J, Dos Santos Silva LA, Rizzoto G, Pinheiro Ferreira JC and Martins MIM (2022) The proAKAP4 concentrations in Nellore bulls’ sperm and their relation to FTAI conception rates results. Animal Reproduction Science. Vol. 247:107156. https://doi.org/10.1016/j.anireprosci.2022.107156
De Almeida MAB, Megumy Tsunokawa HidalgoM, Zito de Moraes FL, Corsi Trautwein LG, Schnitzer J, Dos Santos Silva LA, Rizzoto G, Pinheiro Ferreira JC and Martins MIM (2022) The proAKAP4 concentrations in Nellore bulls’ sperm and its relations to FTAI conception rates results. Rev Bras Reprod Anim. Vol. 46(2)178-179.
Dordas-Perpinyà M, Yanez-Ortiz I, Sergeant N, Mevel V, Bruyas B, Catalán J, Delehedde M, Briand-Amirat L and Miró J (2022) ProAKAP4 concentration is related with sperm motility and motile sperm subpopulations in frozen-thawed horse semen. Animals. Vol:12:3417. https://doi.org/10.3390/ani12233417
Dordas-Perpinyà M, Sergeant N, Ruelle I, Bruyas JF, Charreaux C, Michaud S, Carracedo S, Catalán J, Miró J, Delehedde M and Briand-Amirat L (2022) ProAKAP4 semen concentrations as a valuable marker protein of post-thawed semen quality and bull fertility: a retrospective study. Veterinary Sciences. Vol. 9 :224. https://doi.org/10.3390/vetsci9050224
Dordas-Perpinya M, Yanez I, Catalan J, Eddarkaoui S, Delehedde M, Sergeant N, Bruyas JF, Briand-Amirat L, and Miró J (2022) ProAKAP4 concentrations in stallion and donkey: Comparison with kinetic parameters, motility and concentration. Reproduction in Domestic Animals. Vol. 57(1):61
Dordas-Perpinyà M, Yanez I, Catalan J, Delehedde M, Sergeant N, Bruyas JF, Briand-Amirat L and Miró J (2022) ProAKAP4 correlation with motion parameters and spermatozoa subpopulations in stallion. Reproduction in Domestic Animals. Vol. 57(4):60-61
Fatet A, Sergeant N, Dordas-Perpinyà M, Drouet B, Ponthoreau O, Carracedo S, Bruyas JF, Thorin C, Delehedde M and Briand-Amirat L (2022) Sperm-specific protein proAKAP4 as a marker to evaluate sperm quality and fertility of Alpine and Saanen Bucks. Reproduction in Domestic Animals. Vol. 57(4):79. https://doi.org/10.1111/rda.14244
Kowalczyk A, Gałeska E and Bubel A (2022) The concentration of proAKAP4 and other indicators of cryopotential of spermatozoa cryopreserved in extender with holothuroidea extract addition. Animals. Vol. 12:521. https:// doi.org/10.3390/ani12040521
Riesco MF, Neila-Montero M, Palacín-Martínez C, Montes-Garrido R, Álvarez R; Boixo JC; De Paz P and Anel L and Anel-López L (2022) Establishment of innovative biomarkers to optimize cooling and cryopreservation protocols in ram sperm. Reproduction in Domestic Animals. Vol. 57(1):101.
Schäfer-Somi S, Colombo M and Luvoni GC (2022) Canine Spermatozoa—Predictability of Cryotolerance Animals. Vol. 12:733. https://doi.org/10.3390/ani12060733
Siena G, Milani C, Contiero B, Maenhoudt C, Robiteau G, Slimani S, Sergeant N, Tiret L, Fontbonne A (2022) Variation of ProAKAP4 concentration among different ejaculates from the same dogs. Reproduction in Domestic Animals. Processings of the. ISCFR-EVSSAR Symposium – Italy 2022 | IVIS. Page 68. https://www.ivis.org/library/iscfr/iscfr-evssar-symposium-italy-2022
Smith MZY, Dilger A, Bryan E, Bytnar G, Choi S, Cook K and Kline K (2022) Preliminary Study of Effects of Centrifugation of Stallion Semen on Motility and Pro-AKAP4 Concentration over 72 Hours Post-collection. Journal of Agricultural Science and Technology. Vol (12): 65-72. https://www.davidpublisher.com/Public/uploads/Contribute/63607c5ca4824.pdf DOI: 10.17265/2161-6256/2022.02.003
Van de Hoek M, Rickard JP and de Graaf SP (2022) Motility Assessment of Ram Spermatozoa. Biology. Vol. 11(12): 1715 (review). https:// doi.org/10.3390/biology11121715
2021
Bastan I and Akcay E (2021) Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker. Andrologia e14164. https:// doi.org/10.1111/and.14164
Bastan I and Akcay E (2021) Prognostic assessment of frozen bull semen with ProAKAP4 biomarker. Abstract Congress. 4th International Health Science and Life Congress:227.
Blommaert D, Sergeant N, Delehedde M, Donnay I, Lejeune JP, Franck T and Serteyn D (2021) First results about ProAKAP4 concentration in stallion semen after cryopreservation in two different freezing media. Cryobiology. Vol. 102:133-135.
Le Couazer D and Bencharif D (2021) Are AKAP4 and proAKAP4 Found in Canine Semen? Preliminary Results. Aspects Nanotechnol Vol. 3(1):70-75
Malo C, Carracedo S, Delehedde M, Sergeant N and Skidmore L (2021) Identification of PROAKAP4 in Dromedary sperm and their correlation with monthly semen parameters. Reproduction & Fertility. Vol.2(4):268-279.
Scovell JM, Bournat JC, Szafran AT, Solis M, Moore J, Rivera A, Chen CH, Zhang J, Wilken N, Seth A, Jorgez CJ (2021) PRSS50 is a testis protease responsible for proper sperm tail formation and function. Development Vol.148 (8): dev197558.
2020
Carracedo S, Loyens A, Eddarkaoui S, Serteyn D, Malo C, Skidmore L, Briand-Amirat L, Barbotin AL, Maurage CA, Delehedde M and Sergeant N (2020) The sperm specific proAKAP4 polypeptide exhibited conserved functions, localizations and metabolism among mammals. Animal Reproduction Science. Vol. 220 :106448 https://doi.org/10.1016/j.anireprosci.2020.106448
Delehedde M, Carracedo S, Duchene B, Demouveaux B, Remy G, Kervoaze G, Aubry S, Gevaert MH, Maurage CA, Jonckheere N, Van Seuningen I, Philippe Gosset G, Desseyn JL, Sergeant N and Pichavant M (2020) Cigarette smoking affects the sperm-specific proAKAP4 concentrations and impairs both spermatogenesis and sperm quality. Andrology. Vol. 8 (Suppl. 1):45-46.
Griffin R, Swegen A, Baker M, John Aitken R, A Skerrett-Byrne D, Silva Rodriguez A, E Martín-Cano F, Nixon B, J Peña F, Delehedde M, Sergeant N, Gibb Z (2020) Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa. Reproduction. 160(5):695-707.
Langlade C, Buff S, Dias C, Commin L (2020) Assessment of Optimized Frozen/Thawed Semen Samples in Canines with the New A-Kinase Anchor Protein 4 Precursor Biomarker. Biopreserv Biobank. 18(5):409-414.
Riesco M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Alvarez M, de Paz P, Anel L (2020) ProAKAP4 as Novel Molecular Marker of Sperm Quality in Ram: An Integrative Study in Fresh, Cooled and Cryopreserved Sperm. Biomolecules. 10(7):1046. doi: 10.3390/biom10071046.
Ruelle I, Sergeant N, Bencharif D, Charreaux F, Thorin C, Michaud S, Dordas-Perpinyà M, Jouy N, Audry S, Maurage CA, Delehedde M and Briand-Amirat L (2020) ProAKAP4 concentrations in semen as a predictive tool of bull fertility: A preliminary study. Reproduction, Fertility and Development. 32(1):199. https://www.publish.csiro.au/RD/RDv32n2Ab145
Sergeant N, Blommaert D, Carracedo S, Franck T, Lejeune JP, Gevaert MH, Aubry S, Maurage CA, Serteyn D and Delehedde M (2020) Assessment of the proAKAP4 concentrations as a pertinent sperm parameter to select extenders and preservatives of stallion semen. Animal Reproduction Science. Vol. 220 :106449.
Sergeant N, Carracedo S, Blommaert D, Aubry S, Jouy N, Lejeune JP, Franck T, Maurage CA, Serteyn D, Buée L and Delehedde M (2020) Proteolysis of proAKAP4 in semen as a regulatory sensor of sperm quality and functionality. Andrology. Vol. 8 (Suppl. 1):44-45.
Per ulteriori informazioni CONTATTACI .